![]() Telomere end-replication problem and cell aging. Levy, M.Z., Allsopp, R.C., Futcher, A.B., Greider, C.W. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. Telomere instability in the male germline. Structure and variability of human chromosome ends. Isolation of high-molecular-weight DNA from mammalian cells using proteinase K and phenol. ![]() Telomere fluorescence measurements in granulocytes and T lymphocyte subsets point to a high turnover of hematopoietic stem cells and memory T cells in early childhood. Very short telomere length by flow fluorescence in situ hybridization identifies patients with dyskeratosis congenita. Genome-wide association identifies OBFC1 as a locus involved in human leukocyte telomere biology. Common variants near TERC are associated with mean telomere length. Association of leukocyte telomere length with circulating biomarkers of the renin–angiotensin–aldosterone system: the Framingham Heart Study. Obesity, cigarette smoking, and telomere length in women. Telomere length and possible link to X chromosome. Nawrot, T.S., Staessen, J.A., Gardner, J.P. Offspring's leukocyte telomere length, paternal age, and telomere elongation in sperm. Leukocyte telomeres are longer in African Americans than in whites: the National Heart, Lung, and Blood Institute Family Heart Study and the Bogalusa Heart Study. The load of short telomeres, estimated by a new method, Universal STELA, correlates with number of senescent cells. Extensive allelic variation and ultrashort telomeres in senescent human cells. ![]() Telomere length measurement by a novel monochrome multiplex quantitative PCR method. Telomere measurement by quantitative PCR. Flow cytometry and FISH to measure the average length of telomeres (flow FISH). Heterogeneity in telomere length of human chromosomes. High-throughput telomere length quantification by FISH and its application to human population studies. Telomeres shorten during ageing of human fibroblasts. Telomere biology in healthy aging and disease. Oeseburg, H., de Boer, R.A., van Gilst, W.H. Telomeres and human disease: ageing, cancer and beyond. A skilled technician can measure TRF length in ∼130 samples per week.īlasco, M.A. The method also provides parameters of telomere length distribution in each DNA sample, which are useful in answering questions beyond those focusing on the mean length of telomeres in a given sample. Although precise and highly accurate, the method requires a considerable amount of DNA (3 μg per sample) and it measures both the canonical and noncanonical components of telomeres. After extraction, DNA is inspected for integrity, digested, resolved by gel electrophoresis, transferred to a membrane, hybridized with labeled probes and exposed to X-ray film using chemiluminescence. However, the method can be adapted for telomere length measurements in other cells whose telomere lengths are within its detection boundaries. We use this approach primarily for epidemiological studies that examine leukocyte telomere length. In this protocol we describe a method to obtain telomere length parameters using Southern blots of terminal restriction fragments (TRFs).
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